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S. S. Sadekar , V. B. Chauhan , R. L. Shirole , B. U. Salve , V. S. Kasture , S. B. Kasture

Abstract

Objective: The objective of this study was to investigate the effect of acetone soluble fraction of methanolic extract of leaves of Vitex negundo on dopaminergic function. Methods: The effect of acetone soluble fraction of methanolic extract of leaves of Vitex negundo (50 and 100 mg/kg, i.p.) was studied on haloperidol-induced catalepsy in mice, amphetamine-induced stereotyped behavior in rats and dopamineinduced contraction of isolated vas deferens preparation of rat. Results: The acetone soluble fraction of methanolic extract of V. negundo significantly potentiated haloperidol-induced catalepsy, antagonized dose dependent amphetamine- induced stereotyped behavior, and also antagonized dopamine-induced contractions of rat vas deferens. Conclusion: The results suggest that the methanolic extract of Vitex negundo possessed antidopaminergic principles.

Keywords

Antidopaminergic, Vitex negundo, Amphetamine, Haloperidol

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Shiv Om ¹, V. B. Chauhan ¹, S. B. Sarode ², P. M. Wayazade ³

Affiliations
1 Deptt. of Mycology and Plant Pathology, Banaras Hindu University, Varanasi (U.P.), IN
2 Deptt. of Genetics and Plant Breeding, Banaras Hindu University, Varanasi (U.P.), IN
3 Deptt. of Agronomy, Banaras Hindu University, Varanasi (U.P.), IN

Abstract

The experimental material consisted of 82 pigeonpea genotypes of medium to long duration. Out of 82 genotypes, 22 genotypes were screened for sterility mosaic by using Leaf stapling technique and petiole grafting technique in pots. In field, 82 genotypes were screened by using Infector hedge technique as well as Leaf stapling technique. All the 22 genotypes except TT 701 and SM 03-17 exhibited symptom of sterility mosaic disease when inoculated adopting both leaf stapling and petiole grafting techniques. Out of 82 genotypes tested against sterility mosaic, sixteen were free from disease and grouped as highly resistant, fourteen genotypes were resistant showing 0.1 to 10 per cent disease incidence, while twenty seven genotypes were moderately resistant showing 10.1 to 25 per cent incidence of disease. Rests of the genotypes were susceptible to highly susceptible showing 25.1 to 100 per cent of disease incidence.

Keywords

Pigeonpea, Sterility Mosaic Disease, Petiole Grafting Technique, Leaf Stapling.

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Shiv Om ¹, V. B. Chauhan ¹, S. B. Sarode ², P. M. Wayazade ³

Affiliations
1 Department of Mycology and Plant Pathology, Banaras Hindu University, Varanasi (U.P.), IN
2 Department of Genetics and Plant Breeding, Banaras Hindu University, Varanasi (U.P.), IN
3 Department of Agronomy, Banaras Hindu University, Varanasi (U.P.), IN

Abstract

The experiment was conducted in pots (30 cm in diameter.) which were filled with field soil. Ten selfed seeds of each of 11 genotypes of pigeonpea were sown in three pots at different dates viz., 15^th September 2003, 25^th November 2003 and 16^th January 2004 to know the effect of different dates of sowing on the first symptom appearance and incidence of disease. Pigeonpea genotypes susceptible to sterility mosaic, exhibited symptoms of disease irrespective of three different dates of sowing. The highly resistant genotypes like ICP 7035 and ICP 8862 were free from sterility mosaic at all different dates of sowing. The incidence of disease in all genotypes sown in September was significantly higher as compared to incidence of disease exhibited by genotypes sown in November and January. The period required for first symptom appearance was significantly shorter in September sowing crop as compared to crop grown in November and January.

Keywords

Pigeonpea, Sterility Mosaic.

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